| 名稱(chēng) | c-Kit N822K/BaF3 |
| 型號(hào) | CBP73279 |
| 報(bào)價(jià) |  |
| 特點(diǎn) | c-Kit N822K/BaF3,母細(xì)胞：BaF3 KIT N822K 位于 Kit 蛋白（PMID：24205792）的蛋白激酶結(jié)構(gòu)域中的激活環(huán)內(nèi)。 N822K 導(dǎo)致 Kit 的組成型激活，增加 Erk1/2 和 Stat3 磷酸化，在培養(yǎng)中轉(zhuǎn)化，，導(dǎo)致 Kit 錯(cuò)誤定位到內(nèi)溶酶體，并已被確定為次要與伊馬替尼耐藥相關(guān)的突變。 |
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Email： zhangxiangwen@cobioer.com
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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73279c-Kit N822K/BaF3
- 詳細(xì)內(nèi)容
CBP73279 | |
| I. Introduction | |
Cell Line Name： | c-Kit N822K/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
Mycoplasma Status: | Negative |
| II.Background | |
KIT N822K lies within the activation loop in the protein kinase domain of the Kit protein (PMID: 24205792). N822K results in constitutive activation of Kit, increased Erk1/2 and Stat3 phosphorylation, is transforming in culture (PMID: 24205792, PMID: 31484543), leads to mislocalization of Kit to endolysosomes (PMID: 31484543), and has been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160). | |
| III. Representative Data | |
1.WB of c-Kit N822K/BaF3
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2. Sanger of c-Kit N822K/BaF3
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT N822K(C4). | |
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